Key result
Fasting for up to 9 hours in a rat model of NASH caused a time-dependent increase in hepatic triglycerides and a decrease in serum free fatty acids, but did not affect total cholesterol or liver histopathology.
Why the study?
Fasting prior to lipid profile evaluation has recently been thought unnecessary for most individuals, prompting investigation into its impact in nonalcoholic steatohepatitis.
Does fasting time affect serum and hepatic lipid profiles in a Sprague-Dawley rat model of nonalcoholic steatohepatitis?
Does fasting time affect serum and hepatic lipid profiles in a Sprague-Dawley rat model of nonalcoholic steatohepatitis?
Excessive fasting before blood or hepatic tissue sampling can be avoided for evaluating several parameters in non-NASH and NASH model rats, as fasting alters some lipid profiles but not others.
Fasting alters lipid profiles in NASH rat models; leaves open whether nonfasting sampling is reliable in patients.
Nonalcoholic steatohepatitis (NASH) is associated with several cardiovascular risk factors, including atherogenic dyslipidemia. Recently, fasting prior to lipid profile evaluation has been thought to be unnecessary for most individuals. We investigated the impact of fasting for up to 9 h on the serum and hepatic lipid profiles in Sprague-Dawley (SD) rats of dietary-induced NASH model in comparison to SD rats fed a normal diet. In both groups, fasting affected the serum and hepatic triglyceride (TG), serum free fatty acid (FFA) and leptin levels, histopathologically assessed hepatocyte ballooning, and hepatic mRNA expression levels of several genes related to lipid metabolism. In contrast, the serum adiponectin and aminotransferase levels, serum and hepatic total cholesterol contents, and liver histopathological findings of hepatic steatosis, lobular inflammation and fibrosis were not influenced by fasting. A significant fasting time-dependent reduction was seen in the serum TG level only in the normal SD rats group. Regarding the hepatic TG level, a significant fasting time-dependent increase was seen only in the NASH model rat group. A significant fasting time-dependent reduction was also seen in the serum FFA level only in the NASH model rat group. Our present results indicate that excessive fasting can be avoided before blood or hepatic tissue sampling for the evaluation of several parameters in non-NASH and/or NASH model rats. Further investigations are needed in humans to determine whether excessive fasting before blood or hepatic tissue sampling can be avoided in both healthy individuals and NASH patients.
No takes yet. Share an insight, caveat, or question.
Omagari et al. (2022) studied Nonalcoholic steatohepatitis (NASH) (n=32). Fasting vs. Non-fasting state was evaluated on Serum and hepatic lipid profiles and histopathology. Fasting for up to 9 hours in a rat model of NASH caused a time-dependent increase in hepatic triglycerides and a decrease in serum free fatty acids, but did not affect total cholesterol or liver histopathology.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: