January 31, 2011Proceedings of the National Academy of Sciences

Lethal Arg9Cys phospholamban mutation hinders Ca 2+ -ATPase regulation and phosphorylation by protein kinase A

View Full Paper

Ask AI

Bookmark

Population

In vitro biochemical models and live AAV-293 cells expressing wild-type and R9C mutant phospholamban (PLN)

Comparison

R9C mutation in phospholamban under reducing and… vs Wild-type phospholamban (PLNwt)

Design

Preclinical

Authors

KHKim N. HaUniversity of Minnesota LMLarry R. MastersonUniversity of Minnesota ZHZhanjia HouLoyola University Chicago

Discussion

Member takes

Implication

Hypothesis-generating for redox modulation in PLN cardiomyopathy; leaves open human translation and therapeutic targeting.

Structured PICO

Population

In vitro biochemical models and live AAV-293 cells expressing wild-type and R9C mutant phospholamban (PLN)

Intervention

R9C mutation in phospholamban (PLN) under reducing and oxidizing conditions

Comparator

Wild-type phospholamban (PLNwt)

Outcome

Phosphorylation by PKA-C, PLN monomer-pentamer equilibrium (oligomerization), and SERCA regulation/activitysurrogate

The lethal R9C mutation in phospholamban causes dilated cardiomyopathy by stabilizing the pentameric assembly, preventing SERCA regulation and PKA phosphorylation, especially under oxidative stress.

Cite This Study

Ha et al. (2011) studied this question.

synapsesocial.com/papers/6a9588e31fe04514d10f6411 https://doi.org/10.1073/pnas.1013987108

View Full Paper

Ask AI

Bookmark

Lethal Arg9Cys phospholamban mutation hinders Ca 2+ -ATPase regulation and phosphorylation by protein kinase A

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Lethal Arg9Cys phospholamban mutation hinders Ca 2+ -ATPase regulation and phosphorylation by protein kinase A

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider