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The acid hematein test of Baker was simplified by the following method: Tissues were fixed in either formal-calcium or formal-calcium-cadmium, frozen-sectioned in a cryostat, cbromatized with potassium dichromate, washed in water, stained in acid hematein solution, washed in water again, differentiated in borax-ferricyanide, dehydrated in alcohols, cleared in xylene, and finally mounted in balsam. The process requires only 2 days instead of the 6 days necessary for the original test of Baker. The technique stains phospholipid in mitochondria and in other cellular elements, and is recommended especially for mitochondria
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Samuel H. Hori (1963) studied this question.
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