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August 1, 2023Journal of Medical VirologyOpen Access

Co‐delivery of a trimeric spike DNA and protein vaccine with aluminum hydroxide enhanced Th1‐dominant humoral and cellular immunity against SARS‐CoV‐2

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Authors

HLHung‐Chun LiaoMHMin‐Syuan HuangFCFang‐Feng Chiu

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Overview

Preclinical study demonstrates enhanced Th1-biased immunity and rapid viral clearance in hamsters, indicating a potent combined vaccine strategy against SARS-CoV-2.

Key Points

  • To evaluate the immunogenicity and protective efficacy of co-delivering a trimeric spike protein subunit vaccine with a spike-encoding DNA vaccine formulated with aluminum hydroxide.
  • Formulated recombinant spike-trimer protein with an aluminum hydroxide adjuvant and co-delivered it with a spike-encoding DNA plasmid.
  • Assessed antigen-specific antibody titers, neutralizing antibodies, IgG2a/IgG1 isotype ratios, cytokine profiles, and CD8+ T-cell responses.
  • Challenged immunized Syrian hamsters with SARS-CoV-2 to monitor changes in body weight and quantify lung viral load on day 3 post-infection across experimental arms.
  • The co-delivered vaccine elicited strong CD8+ T-cell responses, elevated neutralizing antibody titers, and a Th1-biased immune profile.
  • At day 3 post-challenge, viral titers in the lungs were undetectable (tissue culture infectious dose < 10) in hamsters receiving the combined vaccine, compared to 10^9 in the PBS group, 10^7 with DNA alone, and 10^6 with protein in alum.
  • Hamsters receiving the combined protein and DNA formulation maintained body weight following challenge, whereas animals receiving DNA alone or the PBS control experienced significant weight reduction.

Cite This Study

Liao et al. (2023) studied this question.

synapsesocial.com/papers/6a958f1fa15fb1352cb303ddhttps://doi.org/10.1002/jmv.29040
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