Key result
Expression of both the coxsackie-adenovirus receptor and alphavbeta5 integrin in an Epstein-Barr virus-transformed B-lymphoblastoid cell line enabled susceptibility to adenovirus infection.
Engineering B cells to express coxsackie-adenovirus receptor and alphavbeta5 integrin allows for successful adenovirus infection, enabling gene modification in these typically resistant cells.
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Does not support clinical B-cell gene therapy; leaves open whether dual receptor expression enables adenoviral transduction in primary lymphocytes.
Human lymphocytes are resistant to genetic modification, particularly from recombinant adenoviruses, thus hampering the analysis of gene function using adenoviral vectors. This study engineered an Epstein-Barr virus-transformed B-lymphoblastoid cell line permissive to adenovirus infection and elucidated key roles for both the coxsackie-adenovirus receptor and alphavbeta5 integrin in mediating entry of adenoviruses into these cells. The work identified a strategy for engineering B cells to become susceptible to adenovirus infection and showed that such a strategy could be useful for the introduction of genes to alter lymphoblastoid-cell gene expression.
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Richardson et al. (2005) studied Adenovirus infection of B lymphocytes. Expression of coxsackie-adenovirus receptor and alphavbeta5 integrin was evaluated on Susceptibility to adenovirus infection. Expression of both the coxsackie-adenovirus receptor and alphavbeta5 integrin in an Epstein-Barr virus-transformed B-lymphoblastoid cell line enabled susceptibility to adenovirus infection.
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