Normalisation of a biarticular muscle EMG signal using a submaximal voluntary contraction: Choice of the standardised isometric task for the rectus femoris, a pilot study

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BACKGROUND: Electromyography (EMG) signal amplitude is often altered by factors related to the participants and the measurement system. To overcome this issue, a normalisation of the EMG signal amplitude can be performed. Recently, it has been demonstrated that a submaximal voluntary contraction (subMVC) normalisation approach, inspired by grade 3 of manual muscle testing, could produce reliable results. However, rectus femoris (RF) normalisation resulted in low reliability. While the normalisation task chosen for this biarticular muscle was to maintain a knee extension against gravity (ISO-K), a hip flexion isometric task (ISO-H) could also be applied. RESEARCH QUESTION: This pilot study aimed to assess the impact of the normalisation task on the RF EMG signal quality and related intra-rater within-day reliability during ISO-K and ISO-H, and intra-rater between-day reliability of the EMG signal amplitude during gait. METHODS: Twenty-four asymptomatic participants were asked to perform ISO-K and ISO-H tasks with both legs and then to walk at self-spontaneous speed, in two identical sessions one week apart. A wireless EMG system was used to record the EMG signal of bilateral RF during each task. RESULTS: Signal-to-noise ratio during ISO-K and ISO-H was ≥ 15 dB in respectively 51% and 98% of all task repetitions. Intra-rater within-day reliability was acceptable using ISO-K (ICC = 0.71 (0.57; 0.83)) with high %SEM of 35%, and excellent using ISO-H (ICC = 0.94 (0.90; 0.96)) with high %SEM of 34%. Intra-rater between-day reliability during gait was acceptable using ISO-K (ICC = 0.74 (0.61; 0.81)) with a high %SEM of 49%, and excellent using ISO-H (ICC = 0.87 (0.76; 0.93)) with a high %SEM of 38%. SIGNIFICANCE: The reliability (ICC) of RF EMG signal normalisation was higher using ISO-H than using ISO-K. However, even if signal-to-noise ratio was notably improved using ISO-H, %SEM remains high whatever the normalisation task used. Some additional improvements might thus still be needed to obtain a normalisation protocol allowing more reproducible measurements.