Experiments were done to determine if cell migration is involved in cartilage differentiation in the embryonic chick limb bud. Blocks of tissue labeled with tritiated thymidine or with S‐35 sulfate were isolated surgically from early stage 22 limb buds or from the chondrogenic areas of stage 23 or stage 24 limb buds and implanted in unlabeled limb buds of embryos growing in ovo. The host embryos were allowed to develop, the limbs containing the labeled implants were fixed, and the effect of the development of the host on the implanted tissue and the effect of the implant on the development of the host was examined by autoradiography. The implanted blocks appeared to be integrated into the host limbs and to develop harmoniously with the host tissue. No evidence was obtained for any of the following events: (1) movement of cells out of the implanted block of tissue, (2) movement of host cells through the implanted block, or (3) interference by the implanted block with the normal development of the host. It is concluded that cell migration is not involved in the differentiation of cartilage in the embryonic chick limb bud.
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Robert L. Searls (1967) studied this question.