July 15, 2001Journal of VirologyOpen Access

Essential Role of Cyclization Sequences in Flavivirus RNA Replication

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Population

BHK cells transfected with mosquito-borne Kunjin virus replicon RNA

Comparison

Mutations in the putative cyclization sequences… vs Wild-type Kunjin virus replicon RNA

Design

Preclinical

Follow-up

up to 48 hours

Authors

AKAlexander A. KhromykhThe University of Queensland HMHedije MekaImperial College London KGK. J. GuyattRoyal Children's Hospital

Discussion

Member takes

Implication

5'-3' cyclization base-pairing is required for Kunjin virus replication; leaves open targeted antiviral approaches pending mammalian validation.

Structured PICO

Population

BHK cells transfected with mosquito-borne Kunjin virus replicon RNA

Intervention

Mutations in the putative cyclization sequences (5' or 3') either separately or simultaneously (compensatory mutations)

Comparator

Wild-type Kunjin virus replicon RNA

Outcome

RNA replication competence (detected by immunofluorescence for NS3 protein and Northern blot analysis)

Base-pairing between 5' and 3' complementary sequences is essential for the replication of mosquito-borne Kunjin virus RNA.

Limitations

Large size of the Flavivirus RNA (11 kb) precludes whole genome secondary structure analysis at present

Cite This Study

Khromykh et al. (2001) studied this question.

synapsesocial.com/papers/6a95abe30c522c5e19a8e90e https://doi.org/10.1128/jvi.75.14.6719-6728.2001

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Implication

5'-3' cyclization base-pairing is required for Kunjin virus replication; leaves open targeted antiviral approaches pending mammalian validation.

Essential Role of Cyclization Sequences in Flavivirus RNA Replication

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Essential Role of Cyclization Sequences in Flavivirus RNA Replication

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider