Key result
An optimized transcription-regulating motif was engineered with a reduced length and showed a 4-fold activity increase in relation to the native RNA motif, enhancing transcription of the 3a gene by 5-fold in infectious TGEV.
Population
Baby hamster kidney cells stably transformed with the porcine amino peptidase N gene and with the Sindbis…
Comparison
Mutagenesis of transcription-regulating motifs… vs Wild-type or reference replicons.
Design
Preclinical
Authors
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May enable efficient coronavirus expression vectors; leaves open validation beyond the TGEV animal model.
Optimization of the transcription-regulating motif in TGEV significantly enhances subgenomic mRNA transcription, which can be utilized in coronavirus-based expression vectors.
Mateos‐Gómez et al. (2011) studied Coronavirus (TGEV) transcription. Optimized transcription-regulating motif (TRM) vs. Native/wild-type transcription-regulating motif was evaluated on Subgenomic mRNA transcription levels. An optimized transcription-regulating motif was engineered with a reduced length and showed a 4-fold activity increase in relation to the native RNA motif, enhancing transcription of the 3a gene by 5-fold in infectious TGEV.