Key result
Deletion or mutagenesis of the internal p33 recognition element, the replication silencer element, and the 3'-terminal minus-strand initiation promoter reduced CNV replicase activity to a minimal level.
The assembly of the tombusvirus replicase complex critically depends on three viral RNA elements: p33RE, RSE, and gPR.
No takes yet. Share an insight, caveat, or question.
Tombusvirus replicase assembly requires these three RNA elements; leaves open their targeting for antiviral strategies.
Panaviene et al. (2005) studied Tombusvirus replication. Mutagenesis of viral RNA sequences (p33RE, RSE, gPR) vs. Wild-type viral RNA was evaluated on In vitro replicase activity. Deletion or mutagenesis of the internal p33 recognition element, the replication silencer element, and the 3'-terminal minus-strand initiation promoter reduced CNV replicase activity to a minimal level.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: