What question did this study set out to answer?

To determine the full-length genomic sequence and coreceptor utilization of a novel HIV-1 A/J recombinant isolate exhibiting complex pol gene mosaicism.

July 1, 2000AIDS Research and Human Retroviruses

Sequence Note: HIV Type 1 A/J Recombinant with a Pronounced pol Gene Mosaicism

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Authors

VNVladimir NovitskyBrown University SGSarah GaolekweUniversity of Botswana MMMary Fran McLaneHarvard University

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Implication

Sequence analysis reveals a replication-competent dual-tropic HIV-1 A/J recombinant in a clinical isolate, highlighting complex pol gene mosaicism and genetic diversity.

Key Points

To determine the full-length genomic sequence and coreceptor utilization of a novel HIV-1 A/J recombinant isolate exhibiting complex pol gene mosaicism.
Isolated HIV-1 strain 98BW21 from a 35-year-old female in Botswana using short-term donor peripheral blood mononuclear cell (PBMC) enrichment.
Amplified, cloned, and sequenced the near full-length viral genome (clone 98BW21.17, 9,103 base pairs from the PBS site to the 3' LTR U5 region).
Conducted phylogenetic analyses across the genome against HIV-1 groups M, N, and O, and subtypes A through K.
Clone 98BW21.17 showed a subtype J backbone mosaic with subtype A segments, but the subtype origin of two pol regions (p51 reverse transcriptase and integrase) could not be identified.
The recombinant exhibited distinct recombination patterns compared to known AGJ/AGIJ isolates (BFP90 and 95ML84).
Phenotypic analysis confirmed replication competence and dual CXCR4/CCR5 coreceptor tropism.

Cite This Study

Novitsky et al. (2000) studied this question.

synapsesocial.com/papers/6a95ae5a5e25f9e817ffac5b https://doi.org/10.1089/08892220050058434

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Implication

Sequence analysis reveals a replication-competent dual-tropic HIV-1 A/J recombinant in a clinical isolate, highlighting complex pol gene mosaicism and genetic diversity.

Sequence Note: HIV Type 1 A/J Recombinant with a Pronounced pol Gene Mosaicism

Discussion

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Implication

Key Points

Cite This Study

Sequence Note: HIV Type 1 A/J Recombinant with a Pronounced pol Gene Mosaicism

Discussion

Member takes

Implication

Key Points

Cite This Study

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