Elevation of cAMP is required for down-regulation, but not agonist-induced desensitization, of endogenous dopamine D1 receptors in opossum kidney cells. Studies in cells that stably express a rat cAMP phosphodiesterase (rPDE3) cDNA

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Population

Opossum kidney cells

Comparison

Pretreatment with dopamine (1 microM) for 1 h vs Wild-type (WT) OK cells

Design

Preclinical

Follow-up

1 hour

Authors

Discussion

Indicates cAMP necessity differs for D1 desensitization versus down-regulation in renal cells; leaves open extension to in vivo models.

Population

Opossum kidney (OK) cells (wild-type and clones C, H, and N transfected with rat cAMP phosphodiesterase cDNA [rPDE3])

Intervention

Pretreatment with dopamine (1 microM) for 1 h

Comparator

Wild-type (WT) OK cells

Outcome

Desensitization (reduction in maximal increase in cAMP) and down-regulation (receptor loss) of D1 dopamine receptorssurrogate

In opossum kidney cells, cAMP accumulation is required for dopamine-induced down-regulation of D1 receptors, but not for their desensitization.

Bates et al. (1993) studied this question.

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