Population
Clonal viral populations of HIV-1 (derived from HIV-1-61 isolate from a 4-year-old boy) in MT-4 cell cultures
Comparison
15 serial passages at a low multiplicity of… vs 15 serial passages at a higher multiplicity of…
Design
Preclinical
Follow-up
15 serial passages
Authors
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Low-MOI HIV-1 passage promotes variants in vitro; leaves open applicability to in vivo evolution absent immune pressure.
Dilute passage of HIV-1 in cell culture significantly promotes the expression of genetic and phenotypic variants, demonstrating that changes previously observed in vivo can occur in a constant in vitro environment without immune selection.
Sánchez‐Palomino et al. (1993) studied this question.
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