What question did this study set out to answer?

To determine whether peripheral blood mononuclear cells from individuals with atopic dermatitis exhibit autologous cytotoxic activity against skin fibroblasts.

April 1, 1982The Journal of Immunology

Cell-mediated cytotoxicity against skin fibroblasts in atopic dermatitis.

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To determine whether peripheral blood mononuclear cells from individuals with atopic dermatitis exhibit autologous cytotoxic activity against skin fibroblasts.
Assessed cytotoxicity by measuring chromium-51 release from cultured autologous and allogeneic skin fibroblasts incubated with peripheral blood mononuclear cells.
Included 12 patients with atopic dermatitis, 6 patients with other diffuse skin diseases, and 8 healthy controls (N=26).
Characterized effector cell subpopulations using plastic adherence, E-rosette fractionation, aggregated IgG inhibition, and complement-dependent lysis with anti-T11, anti-M1, and anti-T3 monoclonal antibodies.
Mean chromium-51 release in atopic dermatitis patients (24.0%) was significantly higher than in patients with other diffuse skin diseases (6.8%) and healthy controls (9.2%, p < 0.01).
Cytotoxicity was mediated by nonadherent, surface immunoglobulin-negative cells and markedly diminished by complement lysis with anti-T11 or anti-M1 antibodies, while anti-T3 treatment enriched cytotoxic activity.
The elevated cytotoxic response against skin fibroblasts occurred independently of serum factors and did not correlate with generalized natural killer cell activity against K562 targets.

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Implication

Comparative study reveals heightened mononuclear cell cytotoxicity against skin fibroblasts in atopic dermatitis, indicating cell-mediated immune mechanisms in skin pathology.

Cite This Study

Leung et al. (1982) studied this question.

synapsesocial.com/papers/6a95b4e44a90f9fdf309e0d9 https://doi.org/10.4049/jimmunol.128.4.1736

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Implication

Comparative study reveals heightened mononuclear cell cytotoxicity against skin fibroblasts in atopic dermatitis, indicating cell-mediated immune mechanisms in skin pathology.

Cell-mediated cytotoxicity against skin fibroblasts in atopic dermatitis.

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Discussion

Implication

Cite This Study

Cell-mediated cytotoxicity against skin fibroblasts in atopic dermatitis.

Key Points

Discussion

Implication

Cite This Study

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