What question did this study set out to answer?

To fabricate hybrid gelatin-poly-l-lactide electrospun scaffolds and establish non-invasive optical imaging techniques to evaluate their composition, structural properties, and cell interactions.

January 1, 2018Journal of Materials Chemistry BOpen Access

Non-invasive characterization of hybrid gelatin:poly- l -lactide electrospun scaffolds using second harmonic generation and multiphoton imaging

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Authors

GPGermano PiccirilloUniversity of Tübingen MDMauro V. DitarantoUniversity of Basilicata NFNora Feuerer

Discussion

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Implication

Experimental study demonstrates non-invasive optical characterization and cell tracking on hybrid gelatin-PLA electrospun scaffolds, highlighting label-free monitoring tools for tissue engineering.

Key Points

To fabricate hybrid gelatin-poly-l-lactide electrospun scaffolds and establish non-invasive optical imaging techniques to evaluate their composition, structural properties, and cell interactions.
Fabricated hybrid gelatin-poly-l-lactide (PLA) fibrous scaffolds via electrospinning from a fluorinated solvent followed by chemical cross-linking.
Characterized polymer composition, cross-linking effectiveness, and fiber structure using Raman microspectroscopy, multiphoton microscopy, and second harmonic generation (SHG).
Seeded human dermal fibroblasts on the scaffolds and performed label-free fluorescence lifetime imaging microscopy (FLIM) to image cell growth and discriminate cells from matrix fibers.
Raman microspectroscopy confirmed effective cross-linking and quantitatively verified increasing PLA content within the hybrid scaffolds.
Electrospun gelatin exhibited collagen-like SHG signals that vanished in pure gelatin upon cross-linking but remained preserved in hybrid gelatin-PLA scaffolds.
Multiphoton imaging and FLIM successfully tracked cellular growth of human dermal fibroblasts and reliably differentiated the seeded cells from the surrounding scaffold material.

Cite This Study

Piccirillo et al. (2018) studied this question.

synapsesocial.com/papers/6a95b76439cc87fe25d032e3 https://doi.org/10.1039/c8tb02026d

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Implication

Experimental study demonstrates non-invasive optical characterization and cell tracking on hybrid gelatin-PLA electrospun scaffolds, highlighting label-free monitoring tools for tissue engineering.

Non-invasive characterization of hybrid gelatin:poly- l -lactide electrospun scaffolds using second harmonic generation and multiphoton imaging

Discussion

Member takes

Implication

Key Points

Cite This Study

Non-invasive characterization of hybrid gelatin:poly- l -lactide electrospun scaffolds using second harmonic generation and multiphoton imaging

Discussion

Member takes

Implication

Key Points

Cite This Study

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