Key result
Physical exercise alleviated cardiac remodelling and improved cardiac function in mice with diabetic cardiomyopathy by upregulating miR-486a-5p, which directly suppressed Mst1 expression and inhibited cardiomyocyte apoptosis.
Why the study?
Physical exercise mitigates cardiac remodelling in diabetic cardiomyopathy, but the molecular mechanisms of this protection and its association with Mst1 inhibition remain unclear.
Does physical exercise improve cardiac remodeling in diabetic cardiomyopathy in a mouse model?
Does physical exercise improve cardiac remodeling in diabetic cardiomyopathy in a mouse model?
Physical exercise mitigates cardiac remodeling and apoptosis in diabetic cardiomyopathy through the miR-486a-5p-mediated inhibition of Mst1.
Answers are grounded in this paper and cite their sources.
Hypothesis-generating for exercise in diabetic cardiomyopathy; leaves open human translation and clinical adoption.
OBJECTIVES: Physical exercise has emerged as an effective therapy to mitigate cardiac remodelling in diabetic cardiomyopathy (DCM). The results of our previous studies revealed mammalian sterile 20-like kinase 1 (Mst1) is a key regulator of the progression of DCM. However, the precise molecular mechanism of physical exercise-induced cardiac protection and its association with Mst1 inhibition remain unclear. MATERIALS AND METHODS: Wildtype and Mst1 transgenic mice were challenged with streptozotocin (STZ) to induce experimental diabetes and were divided into sedentary and exercise groups. The DCM phenotype was evaluated by echocardiography, Masson's trichrome staining, TUNEL and immunoblotting analyses. The exercise-regulated miRNAs targeting Mst1 were predicted by bioinformatic analysis and later confirmed by RT-qPCR, immunoblotting, and dual-luciferase reporter assays. In addition, cultured neonatal mouse cardiomyocytes were subjected to simulate diabetes to elucidate the underlying mechanisms. RESULTS: Compared to the sedentary diabetic control, physical exercise inhibited Mst1 and alleviated cardiac remodelling in mice with DCM, as evidenced by decreases in the left ventricular end-systolic internal dimension (LVESD) and left ventricular end-diastolic internal dimension (LVEDD), increases in the left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS), attenuation of collagen deposition, and the suppression of apoptosis. Bioinformatic analysis and apoptosis assessments revealed exercise exerted protective effects against DCM through miR-486a-5p release. Moreover, luciferase reporter assays confirmed miR-486a-5p directly suppressed the expression of Mst1, thereby inhibiting the apoptosis of cardiomyocytes subjected to high glucose treatment. CONCLUSION: Physical exercise inhibits cardiac remodelling in DCM, and the mechanism is associated with miR-486a-5p release-induced Mst1 inhibition.
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Sun et al. (2021) studied Diabetic cardiomyopathy (n=50). Physical exercise (treadmill running) vs. Sedentary control was evaluated on Cardiac remodelling and function (LVEF, LVFS, LVESD, LVEDD, apoptosis, fibrosis) (p=<0.05). Physical exercise alleviated cardiac remodelling and improved cardiac function in mice with diabetic cardiomyopathy by upregulating miR-486a-5p, which directly suppressed Mst1 expression and inhibited cardiomyocyte apoptosis.
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