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Hypophysectomized adult newts exhibited 98% survival and limb regeneration at 23 days post‐hypophysectomy when injected intraperitoneally every other day with prolactin (0.015 U/newt) and kept continuously in aquaria with 1 × 10−7 concentration of thyroxine. Thyroxine alone was no more effective than saline injections. Prolactin (1.2 U/newt every other day) alone increased survival and limb regeneration, but less effectively than did the prolactin‐thyroxine combination.
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Connelly et al. (1968) studied this question.
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