Key result
Irbesartan inhibited human Tenon fibroblast migration and reactive oxygen species production, reduced cell numbers, and altered morphology in vitro.
Why the study?
Does irbesartan inhibit human Tenon fibroblast migration and reactive oxygen species production in cell culture?
Does irbesartan inhibit human Tenon fibroblast migration and reactive oxygen species production in cell culture?
Irbesartan demonstrates antifibrotic effects by inhibiting human Tenon fibroblast migration and ROS production in vitro, suggesting potential utility in glaucoma filtration surgery.
Should not yet change glaucoma surgery practice; hypothesis-generating for irbesartan antifibrotic effects in vivo.
PURPOSE: We investigate the effect of angiotensin receptor blockade on the migration of human Tenon fibroblasts (HTF), using irbesartan, an angiotensin II receptor type 1 (AT1R) blocker (ARB) as a potential antifibrotic agent in glaucoma filtration surgery. METHODS: Confluent HTF cultures were scratched with a 1 mL pipette tip and treated with either irbesartan (10, 50, and 100 μg/mL) or angiotensin II (2 μg/mL). The extent of HTF migration up to 30 hours, and cell number and morphology at 72 hours was evaluated. To assess the effect on reactive oxygen species (ROS) level, HTF were treated with either irbesartan (10 μg/mL) or angiotensin II (2 μg/mL) for 24 hours after scratching, and then stained with dihydroethidium (DHE) before evaluation by confocal microscopy. Conversely, angiotensin II increased cell numbers up to 4-fold while retaining cell viability. CONCLUSIONS: Irbesartan inhibited HTF migration and ROS production. It also reduced cell numbers and altered HTF morphology. Angiotensin II increased cell number without altering morphology. This initial study warrants future investigations for further potential antifibrotic effects of this drug. TRANSLATIONAL RELEVANCE: This in vitro study focused on investigations of irbesartan's effects on HTF migration, ROS production, as well as HTF cell numbers and morphology. It suggests a potential therapeutic strategy worth further exploration with a view towards postoperative wound healing modulation in glaucoma filtration surgery.
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Kim et al. (2018) studied Glaucoma filtration surgery (in vitro model). Irbesartan vs. Angiotensin II (2 μg/mL) was evaluated on HTF migration, cell number, morphology, and reactive oxygen species (ROS) level (p=< 0.05). Irbesartan inhibited human Tenon fibroblast migration and reactive oxygen species production, reduced cell numbers, and altered morphology in vitro.
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