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Stimulation of M3 muscarinic receptors in SH-SY5Y cells induced a dose- and time-related increase in Ins(1,4,5)P3 and Ins(1,3,4,5)P4 mass that was dependent upon extracellular Ca2+.
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Stimulation of M3 muscarinic receptors in SH-SY5Y cells increases Ins(1,4,5)P3 and Ins(1,3,4,5)P4 mass in a calcium-dependent manner.
Stimulation of M3 muscarinic receptors expressed by SH-SY5Y cells induced a dose- and time-related increase in the mass of Ins(1,4,5)P3 (basal 38.3 +/- 5.8 pmol/mg of protein) and Ins(1,3,4,5)P4 (basal 6.1 +/- 1.2 pmol/mg of protein). Comparison of radioreceptor mass assays with [3H]inositol labelling showed higher-fold stimulations with the former protocol. The later accumulation of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 mass was dependent upon extracellular Ca2+.
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Lambert et al. (1991) studied SH-SY5Y neuroblastoma cells. Stimulation of M3 muscarinic receptors vs. Basal state was evaluated on Mass of Ins(1,4,5)P3 and Ins(1,3,4,5)P4. Stimulation of M3 muscarinic receptors in SH-SY5Y cells induced a dose- and time-related increase in Ins(1,4,5)P3 and Ins(1,3,4,5)P4 mass that was dependent upon extracellular Ca2+.
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