What question did this study set out to answer?

To solubilize, purify, and characterize the inositol 1,3,4,5-tetrakisphosphate receptor from cerebellar tissue and determine whether it represents a distinct entity from the inositol 1,4,5-trisphosphate receptor.

July 30, 1990FEBS LettersOpen Access

High‐affinity inositol 1,3,4,5‐tetrakisphosphate receptor from cerebellum: solubilization, partial purification and characterization

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Authors

FDFrédéric DoniéUniversity of Tübingen EHE HülserOtto-von-Guericke University Magdeburg GRGeorg ReiserOtto-von-Guericke University Magdeburg

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Implication

Biochemical analysis demonstrates a distinct high-affinity inositol 1,3,4,5-tetrakisphosphate receptor in porcine cerebellum, suggesting an independent role in signal transduction.

Key Points

To solubilize, purify, and characterize the inositol 1,3,4,5-tetrakisphosphate receptor from cerebellar tissue and determine whether it represents a distinct entity from the inositol 1,4,5-trisphosphate receptor.
Solubilized inositol phosphate-binding proteins from porcine cerebellar membrane fractions.
Separated and purified the inositol 1,3,4,5-tetrakisphosphate receptor using heparin-agarose chromatography.
Assessed ligand binding affinity, competition kinetics across related inositol phosphates, pH sensitivity, and calcium dependence.
Purification achieved an approximate 1,000-fold enrichment of the receptor, yielding a dissociation constant (Kd) of 4.6 nM for inositol 1,3,4,5-tetrakisphosphate.
Binding displayed marked selectivity over other inositol phosphates, with an IC50 of 150 nM for Ins 1,4,5,6-P4, 1 µM for Ins-P6, and 5 µM for Ins 1,3,4-P3, while Ins 1,4,5-P3 showed no displacement up to 10 µM.
Receptor binding was maximal at pH 4.5 to 6.0, remained stable across Ca2+ concentrations from 1 nM to 1 mM, and was inhibited by heparin with an IC50 of approximately 2 nM.

Cite This Study

Donié et al. (1990) studied this question.

synapsesocial.com/papers/6a8b2179eee946ed63e3aefa https://doi.org/10.1016/0014-5793(90)81006-a

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Implication

Biochemical analysis demonstrates a distinct high-affinity inositol 1,3,4,5-tetrakisphosphate receptor in porcine cerebellum, suggesting an independent role in signal transduction.

High‐affinity inositol 1,3,4,5‐tetrakisphosphate receptor from cerebellum: solubilization, partial purification and characterization

Discussion

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Implication

Key Points

Cite This Study

High‐affinity inositol 1,3,4,5‐tetrakisphosphate receptor from cerebellum: solubilization, partial purification and characterization

Discussion

Member takes

Implication

Key Points

Cite This Study

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