What question did this study set out to answer?

To characterize inositol 1,4,5-trisphosphate binding sites in brain tissue and examine their regulation by physiological calcium concentrations and pH.

September 1, 1987Journal of Biological ChemistryOpen Access

Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium.

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Authors

PWP F WorleyJohns Hopkins University JBJay M. BarabanJohns Hopkins University SSSurachai SupattaponeDartmouth College

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Implication

Biochemical study reveals that calcium and alkaline pH regulate inositol trisphosphate receptor binding in cerebellar membranes, suggesting physiological control of second messenger signaling.

Key Points

To characterize inositol 1,4,5-trisphosphate binding sites in brain tissue and examine their regulation by physiological calcium concentrations and pH.
Conducted in vitro receptor binding assays on cerebellar membranes to determine binding site density and affinity for inositol 1,4,5-trisphosphate compared with other inositol phosphates.
Assessed the regulatory effects of varying submicromolar calcium concentrations and pH levels on receptor binding.
Cerebellar membranes demonstrated abundant inositol 1,4,5-trisphosphate binding sites (20 pmol/mg protein) with high affinity (KD ≈ 40 nM) and strong selectivity over inositol 1,3,4,5-tetrakisphosphate (Ki ≈ 10 µM) and inositol 1,4-bisphosphate (Ki ≈ 10 µM).
Submicromolar calcium concentrations strongly inhibited inositol 1,4,5-trisphosphate binding with an IC50 of approximately 300 nM.
Receptor binding displayed a sharp increase under slightly alkaline pH conditions.

Cite This Study

Worley et al. (1987) studied this question.

synapsesocial.com/papers/6a6fdd6375498292b7093dd9 https://doi.org/10.1016/s0021-9258(18)45326-4

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Implication

Biochemical study reveals that calcium and alkaline pH regulate inositol trisphosphate receptor binding in cerebellar membranes, suggesting physiological control of second messenger signaling.

Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium.

Discussion

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Implication

Key Points

Cite This Study

Characterization of inositol trisphosphate receptor binding in brain. Regulation by pH and calcium.

Discussion

Member takes

Implication

Key Points

Cite This Study

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