What question did this study set out to answer?

To validate direct ITS2-High Resolution Melting Analysis against standard culture and MALDI-TOF mass spectrometry for identifying Candida species in clinical samples.

August 21, 2015PLoS ONEOpen Access

Validation of High Resolution Melting Analysis (HRM) of the Amplified ITS2 Region for the Detection and Identification of Yeasts from Clinical Samples: Comparison with Culture and MALDI-TOF Based Identification

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Authors

HDHans DuyvejonckUniversity College Ghent PCPiet CoolsGhent University JDJohan DecruyenaereGhent University Hospital

Discussion

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Implication

Diagnostic study demonstrates 83.9% concordance between ITS2 melting analysis and culture with MALDI-TOF in clinical swabs, indicating strong potential for direct yeast detection.

Key Points

To validate direct ITS2-High Resolution Melting Analysis against standard culture and MALDI-TOF mass spectrometry for identifying Candida species in clinical samples.
Tested 347 clinical swab samples (throat, rectal, and vaginal) from gynaecology/obstetrics, intensive care, and haematology wards at Ghent University Hospital, Belgium.
Extracted DNA using NucliSENS easyMAG for direct ITS2-HRM detection and compared outcomes to indirect culture on Candida ID agar with MALDI-TOF identification.
Achieved complete concordance in 83.9% of samples, with identical Candida species detected in 31.1% and no species detected in 52.8%.
Observed discrepancies in 16.1% of samples (major discrepancies: 6.01%), primarily driven by low fungal cell counts or polymicrobial samples.
Direct ITS2-HRM resolved most discrepancies favorably by capturing low-abundance yeasts and Candida robusta missed by traditional agar culture.

Cite This Study

Duyvejonck et al. (2015) studied this question.

synapsesocial.com/papers/6a95c16df3e79ccb8a3194cc https://doi.org/10.1371/journal.pone.0132149

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Implication

Diagnostic study demonstrates 83.9% concordance between ITS2 melting analysis and culture with MALDI-TOF in clinical swabs, indicating strong potential for direct yeast detection.

Validation of High Resolution Melting Analysis (HRM) of the Amplified ITS2 Region for the Detection and Identification of Yeasts from Clinical Samples: Comparison with Culture and MALDI-TOF Based Identification

Discussion

Member takes

Implication

Key Points

Cite This Study

Validation of High Resolution Melting Analysis (HRM) of the Amplified ITS2 Region for the Detection and Identification of Yeasts from Clinical Samples: Comparison with Culture and MALDI-TOF Based Identification

Discussion

Member takes

Implication

Key Points

Cite This Study

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