Using Aflatoxin B1 Regulatory Gene Sequencing to Study Similarity and Difference between Aspergillus flavus Strains, Isolated from Patients with Aspergllosis

Aflatoxins are carcinogenic fungal secondary metabolites produced by Aspergillus flavus and other closely related species, The work in this study presented the first detailed molecular analyses of AFLAR2 gene in the strains of Aspergillus flavus isolated from patient with aspergillosis infections In this study, rapid assessment of 14 isolates of A. flavus was accomplished using an indigenously designed primer pair for the Aflatoxin regulatory gene aflR2 in Polymerase Chain Reaction (PCR). Specificity was assayed in pure culture systems using DNA extracted from 14 different A. flavus straines as PCR template. Positive amplification was achieved only with DNA from 10 A. flavus that produce Aflatoxin B1.