July 1, 1991Journal of Microscopy
Cryoultramicrotomy of muscle: improved preservation and resolution of muscle ultrastructure using negatively stained ultrathin cryosections
Population
Muscle tissue from chicken, rabbit, and fish
Design
Preclinical
Authors
Discussion
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Implication
Enables 3D macromolecular analysis in animal muscle models; leaves open translation to human cardiac tissue.
Structured PICO
P
Population
Muscle tissue from chicken, rabbit, and fish
I
Intervention
Cryoultramicrotomy with negative staining technique
O
Outcome
Preservation of in-vivo order in muscle fibres and resolution of muscle ultrastructuresurrogate
Cryoultramicrotomy with negative staining preserves muscle ultrastructure in 3-D, enabling detailed morphological analysis at the macromolecular level.
Cite This Study
Sjöstróm et al. (1991) studied this question.