Stable hybridoma cell lines secreting monoclonal antibodies to the NY-1 isolate of grapevine leafroll associated closteroviruses (GLRaV) were produced by fusing spleen cells of immunized BALB/c mice and mouse myeloma cell line SP2/0-AG14. The monoclonal antibodies reacted with the NY-1 isolate and other type III isolates, but not with type I, II, and IV isolates. The reactions were the same in five different kinds of enzyme-linked immunosorbent assays (ELISA), immunosorbent electron microscopy, dot-immunoblotting, and Western blotting assays. Sensitivity of the monoclonal antibodies were very good for the detection of the virus in grape leaf tissue in double antibody sandwich ELISA (...)
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John Hu (1990) studied this question.