What question did this study set out to answer?

To investigate whether insulin binds specifically to hypothalamic membranes and localized neuroanatomical brain structures in primates and swine.

March 1, 1983Diabetes

Binding of Insulin by Monkey and Pig Hypothalamus

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Authors

BLBernard R. LandauUniversity of Vermont YTYoshiro TakaokaTeikyo University MAMarc AbramsAbrams Eye Institute

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Implication

Experimental study reveals specific insulin binding localized to hypothalamic regions in monkeys and pigs, indicating direct central nervous system targeting by circulating insulin.

Key Points

To investigate whether insulin binds specifically to hypothalamic membranes and localized neuroanatomical brain structures in primates and swine.
Measured binding kinetics, dissociation rates, and competitive displacement using [125I]insulin and insulin analogs in membrane preparations from monkey and pig hypothalami, cortex, and thalamus.
Perfused an arterial split monkey brain preparation with [125I]insulin alone in one hemisphere and [125I]insulin plus excess unlabeled insulin in the other to localize specific binding via radioautography.
[125I]insulin bound specifically to hypothalamic membranes with first-order dissociation kinetics (half-time of 14 min at 22 °C), exhibiting higher binding in the anterior pig hypothalamus and greater binding overall than in cortex or thalamus.
Analog inhibition matched relative biological activities: desalanine insulin equaled native insulin, while proinsulin and desoctapeptide insulin demonstrated progressively lower inhibition.
Radioautography of perfused monkey brains localized specific blood-borne insulin binding directly to the median eminence, infundibular nucleus, and cerebral microvessels.

Cite This Study

Landau et al. (1983) studied this question.

synapsesocial.com/papers/6a95d01f8372d5bc8453951e https://doi.org/10.2337/diab.32.3.284

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Implication

Experimental study reveals specific insulin binding localized to hypothalamic regions in monkeys and pigs, indicating direct central nervous system targeting by circulating insulin.

Binding of Insulin by Monkey and Pig Hypothalamus

Discussion

Member takes

Implication

Key Points

Cite This Study

Binding of Insulin by Monkey and Pig Hypothalamus

Discussion

Member takes

Implication

Key Points

Cite This Study

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