Who's NEXT-A: Combination of the NEXT-A method and noncatalytic Huisgen reaction enabled us to immobilize proteins onto a solid support. Lectin-immobilized gel made by using this novel method, was subjected to frontal affinity chromatography to detect sugar–lectin interactions. Immobilization of a target protein directly from cell lysates was also demonstrated.
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Ebisu et al. (2009) studied this question.
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