Metabolite Profiling of Compounds from Sargassum polycystum using UPLC-QToF-MS/MS

Using the UPLC-QToF MS/MS equipment, this study seeks to determine the metabolite profile of brown seaweed (Sargassum polycystum) from Sumenep, Madura Island, Indonesia. Using the Solid Phase Extraction (SPE) technique, extracts and fractions were obtained. The samples were subsequently placed within the ACQUITY UPLC H-Class System's MS Xevo G2-S QToF detector (Waters, USA). The samples were separated on an ACQUITY BEH C18 column (1.7 m x 2.1 x 50 mm) with acetonitrile + 0.05% formic acid and water + 0.05% formic acid as mobile phases, with a flow rate of 0.2 ml/min. The results of the UPLC-QToF-MS/MS analysis were processed using MassLynx 4.1 software to obtain chromatogram data and m/z spectra of each detected peak. The detected compounds were further confirmed using the ChemSpider and MassBank online databases.