What question did this study set out to answer?

To review cytochemical localization techniques and their applications for examining endothelial cells and their structural components.

November 1, 1991Journal of Electron Microscopy Technique

Lectin and immunolabeling of microvascular endothelia

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Authors

AMAnthony J. MiliciSarepta Therapeutics (United States)GPGlyn A. PorterYale University

Discussion

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Implication

Methodological review outlines cytochemical localization techniques in microvascular endothelia, highlighting applications for tracing exogenous and endogenous proteins.

Key Points

To review cytochemical localization techniques and their applications for examining endothelial cells and their structural components.
Evaluated tissue processing protocols utilizing L.R. White resin embedding alongside semi-thin and thin cryosectioning.
Applied lectin histochemistry and immunolabeling approaches paired with fluorescence and electron microscopy to detect endothelial targets.
Demonstrated effective cytochemical localization of both perfused exogenous proteins and native endothelial-associated proteins.
Identified trade-offs between resin embedding and frozen sectioning regarding ultrastructural preservation versus antigenic epitope retention.

Cite This Study

Milici et al. (1991) studied this question.

synapsesocial.com/papers/6a95dca935ce0ca9f82507f5 https://doi.org/10.1002/jemt.1060190306

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Implication

Methodological review outlines cytochemical localization techniques in microvascular endothelia, highlighting applications for tracing exogenous and endogenous proteins.

Lectin and immunolabeling of microvascular endothelia

Discussion

Member takes

Implication

Key Points

Cite This Study

Lectin and immunolabeling of microvascular endothelia

Discussion

Member takes

Implication

Key Points

Cite This Study

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