What question did this study set out to answer?

Characterize the transcriptional programs and regulatory gene networks that govern endosperm cell differentiation and tissue compartmentalization in the developing maize kernel.

March 1, 2015The Plant CellOpen Access

RNA Sequencing of Laser-Capture Microdissected Compartments of the Maize Kernel Identifies Regulatory Modules Associated with Endosperm Cell Differentiation

View Full Paper

Ask AI

Bookmark

Authors

JZJunpeng ZhanHuazhong Agricultural University DTDhiraj ThakareRoche (United States)CMChuang MaHeilongjiang University

Discussion

Member takes

Implication

Transcriptomic profiling uncovers compartment-specific gene networks across maize kernel cell types, highlighting regulatory modules that drive endosperm cell differentiation.

Key Points

Characterize the transcriptional programs and regulatory gene networks that govern endosperm cell differentiation and tissue compartmentalization in the developing maize kernel.
Isolated mRNAs from five major cell types of the differentiating endosperm, the embryo, and four maternal compartments of Zea mays kernels using laser-capture microdissection followed by RNA sequencing.
Performed gene coexpression network analysis across kernel compartments to identify cell-type-specific modules and evaluated them for enrichment of temporally regulated and imprinted genes.
Gene expression patterns diverged markedly between filial and maternal compartments, while revealing an unexpected close correlation between the embryo and the endosperm aleurone layer.
Coexpression network analysis resolved distinct modules for specific endosperm cell types with significant enrichment for imprinted and temporally activated genes.
Identified a regulatory module specific to the basal endosperm transfer layer activated downstream of MRP-1, a key transcription factor controlling transfer cell differentiation.

Cite This Study

Zhan et al. (2015) studied this question.

synapsesocial.com/papers/6a9619693d7d987bd901c6aa https://doi.org/10.1105/tpc.114.135657

View Full Paper

Ask AI

Bookmark

Implication

Transcriptomic profiling uncovers compartment-specific gene networks across maize kernel cell types, highlighting regulatory modules that drive endosperm cell differentiation.

RNA Sequencing of Laser-Capture Microdissected Compartments of the Maize Kernel Identifies Regulatory Modules Associated with Endosperm Cell Differentiation

Discussion

Member takes

Implication

Key Points

Cite This Study

RNA Sequencing of Laser-Capture Microdissected Compartments of the Maize Kernel Identifies Regulatory Modules Associated with Endosperm Cell Differentiation

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider