A Protocol to Induce Brown and Beige Adipocyte Differentiation From Murine and Human Adipose-Derived SVF

Figures

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  Figure 1.. Gene expression analysis of brown/beige selective genes in control and differentiated adipocytes. Relative mRNA levels of UCP1, Cidea, Dio2, and PRDM16 in brown-like differentiated adipocytes of stromal vascular fraction (SVF) derived from subcutaneous white adipose tissue (scWAT) of mouse (a) and human (b) (n = 3). 36B4 mRNA expression was used for normalization. Results are expressed as a mean ± SD; different groups were compared via Student’s t-test or by one-way ANOVA; *p-value < 0.05, **p-value < 0.005, ***p-value < 0.001. Primer sequences are available upon request.

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  Figure 2.. Expression of adipogenic markers in brown-like differentiated adipocytes. Relative mRNA levels of PPARγ (a, c) and aP2 (b, d) in mouse and human subcutaneous white adipose tissue (scWAT)-derived stromal vascular fraction (SVF) differentiated into brown-like adipocytes (n = 3). 36B4 mRNA expression was used for normalization. Results are expressed as mean ± SD; different groups were compared via Student’s t-test or by one-way ANOVA; *p-value < 0.05, **p-value < 0.005, ***p-value < 0.001. Primer sequences are available upon request.

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  Figure 3.. Expression of the key brown fat functionality markers in control and differentiated adipocytes. Relative mRNA levels of Elovl3, Mcad, Cpt1α, CytC, and Cox4β in brown-like differentiated adipocytes of stromal vascular fraction (SVF) derived from subcutaneous white adipose tissue (scWAT) of mouse (a) and human (b) (n = 3). 36B4 mRNA expression was used for normalization. Results are expressed as mean ± SD; different groups were compared via Student’s t-test or by one-way ANOVA; *p-value < 0.05, **p-value < 0.005, ***p-value < 0.001. Primer sequences are available upon request.

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  Figure 4.. Brightfield microscopy images of brown-like adipocytes differentiated from subcutaneous white adipose tissue (scWAT) -derived stromal vascular fraction (SVF) of mouse and human (day 8). Ethanol was used as control. Magnification, 20×; scale bar represents 100 μm.