What question did this study set out to answer?

Identify and clone novel opiate receptor gene family members and characterize their alternative splice variants and pharmacological properties.

July 4, 1994FEBS LettersOpen Access

cDNA Cloning of an orphan opiate receptor gene family member and its splice variant

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Authors

JWJia Bei WangCreighton University YIYasuo ImaiDokkyo University APAntonio M. PersicoUniversity of Messina

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Implication

Molecular cloning study reveals a novel orphan opiate receptor gene and splice variant in rat tissues, highlighting structural diversity within the receptor family.

Key Points

Identify and clone novel opiate receptor gene family members and characterize their alternative splice variants and pharmacological properties.
Constructed XOR1S from two partial cDNAs isolated via cDNA homology approaches, and identified a longer variant (XOR1L) using PCR on rat brain, peripheral tissues, and genomic DNA.
Expressed both clones in COS cells to assess radioligand binding and evaluate inhibition of forskolin-stimulated adenylate cyclase.
Deduced XOR1 translation products exhibited 67% to 72% homology with mu 1, delta 1, and kappa 1 opiate receptors, with mRNA expression predominantly localized to the hypothalamus.
Transfected COS cells expressing either XOR1S or XOR1L exhibited neither robust opiate ligand binding nor reproducible inhibition of forskolin-stimulated adenylate cyclase.

Cite This Study

Wang et al. (1994) studied this question.

synapsesocial.com/papers/6a962662bd6feb537a4a22eb https://doi.org/10.1016/0014-5793(94)00557-5

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Implication

Molecular cloning study reveals a novel orphan opiate receptor gene and splice variant in rat tissues, highlighting structural diversity within the receptor family.

cDNA Cloning of an orphan opiate receptor gene family member and its splice variant

Discussion

Member takes

Implication

Key Points

Cite This Study

cDNA Cloning of an orphan opiate receptor gene family member and its splice variant

Discussion

Member takes

Implication

Key Points

Cite This Study

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