ELISA Detection of Various Tomato Spotted Wilt Virus Isolates Using Specific Antisera to Structural Proteins of the Virus

(...)Nineteen isolates were consistently detected in different ELISA tests using various antibodies, while the other 11 isolates either were not detected or were inconsistently detected in these tests. Of the 11 isolates that gave inconsistent results, eight were purified and compared by western blot. They reacted similarly to antibodies to the virion and to anti-26K NP and anti-78K MP antibodies. Most isolates of TSWV from the United States were considered to be closely related serologically. The DAS direct ELISA using antibodies to the whole virions is recommended over the use of antibodies to structural proteins for detecting a diverse selection of isolates of TSWV