Identifying Selective Protein Tyrosine Phosphatase Substrates and Inhibitors from a Fluorogenic, Combinatorial Peptide Library

Protein tyrosine phosphatases are increasingly recognized as enzymes that exhibit exquisite substrate selectivity with important roles in cellular signaling, and have been identified as attractive therapeutic targets in human diseases including autoimmunity, obesity, diabetes, and cancer. A new approach was developed to rapidly and efficiently profile the substrate selectivity of protein tyrosine phosphatase and is described herein.