Proteases and lipases from a number of different species of psychrotrophic bacteria isolated from dairy products are resistant to heat treatments of 77°C for a holding time of 17 s and 140°C for a holding time of 5s. A further treatment combining the 77°C for 17 s heating process with a heat treatment involving a temperature of 55°C for 1 h was also examined. The proteases and, to a lesser extent, the lipases were also resistant to the treatments in combination. The resistance of proteases and lipases to inactivation by a treatment of 55°C for 1 h varied between and within species. Therefore, this treatment may not have a widespread practical application.
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Griffiths et al. (1981) studied this question.
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