Region-Specific Microstructure in the Neonatal Ventricles of a Porcine Model

Figure 1. The anterior (a) and posterior (b) aspects of one-day-old neonatal porcine heart. Post-processed DT-MRIs at 25% fibre density, enabling visualisation of the fibre orientation on the anterior (c) and posterior (d) surface. The regions of interest used to calculate the fractional anisotropy (FA) for the base identified as: (1) base, (2) equator, and (3) apex. Scale bar = 8 mm.

Figure 2. In-plane TPEF/SHG images, with the SHG-channel (green) identifying the collagen fibril distribution, and the TPEF-channel (red) the cardiomyocyte. Both channels were merged to demonstrate the collagen-cardiomyocyte overlapping. Scale bar = 100 µm.

Figure 3. TPEF/SHG microscopy was used to quantify the preferred orientation of the cardiomyocyte and collagen fibril microstructural parameters in the anterior LVFW (n = 5). (a) in-plane preferred orientation; (b) out-plane preferred orientation. (c) in-plane dispersion; (d) out-plane dispersion. (e) in-plane amount; (f) out-plane amount. In-plane and out-plane image-stacks were acquired through the depth of 200-and 1022 µm respectively.

Figure 4. TPEF/SHG microscopy was used to quantify the preferred orientation of the cardiomyocyte and collagen fibril microstructural parameters in the anterior RVFW (n = 5). (a) in-plane preferred orientation; (b) out-plane preferred orientation. (c) in-plane dispersion; (d) out-plane dispersion. (e) in-plane amount; (f) out-plane amount. In-plane and out-plane image-stacks were acquired through the depth of 200-and 1022 µm respectively.

Figure 5. TPEF/SHG microscopy was used to quantify the preferred orientation of the cardiomyocyte and collagen fibril microstructural parameters in the posterior LVFW (n = 5). (a) in-plane preferred orientation; (b) out-plane preferred orientation. (c) in-plane dispersion; (d) out-plane dispersion. (e) in-plane amount; (f) out-plane amount. In-plane and out-plane image-stacks were acquired through the depth of 200-and 1022 µm respectively.

Figure 6. TPEF/SHG microscopy was used to quantify the preferred orientation of the cardiomyocyte and collagen fibril microstructural parameters in the posterior RVFW (n = 5). (a) in-plane preferred orientation; (b) out-plane preferred orientation. (c) in-plane dispersion; (d) out-plane dispersion. (e) in-plane amount; (f) out-plane amount. In-plane and out-plane image-stacks were acquired through the depth of 200-and 1022 µm respectively.

Figure 7. TPEF/SHG microscopy was used to quantify the extent of in-plane and out-plane cardiomyocytes rotation and dispersion in the anterior and posterior aspects of LVFW and RVFW. (a) in-plane rotation; (b) out-plane rotation. (c) in-plane dispersion; (d) out-plane dispersion. In-plane and out-plane image-stacks were acquired through the depth of 200-and 1022 µm respectively.

Figure 8. TPEF/SHG microscopy was used to quantify the extent of in-plane and out-plane collagen fibrils rotation and dispersion in the anterior and posterior aspects of LVFW and RVFW. (a) in-plane rotation; (b) out-plane rotation. (c) in-plane dispersion; (d) out-plane dispersion. In-plane and out-plane image-stacks were acquired through the depth of 200-and 1022 µm respectively.