A micro-technique for measuring complement fixing antibody with cultured Trypanosoma cruzi as antigen in experimental Trypanosoma rhodesiense infection is described. A low titre of complement fixing antibody appeared 2 weeks after the T. rhodesiense infection and rose to a high level in the presence of active infection. T. cruzi offers some advantage over Trypanosoma equiperdum as antigen because of the ease of its in vitro culture.
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Seah et al. (1970) studied this question.
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