Anti-inflammatory activity of berberine in non-alcoholic fatty liver disease via the Angptl2 pathway

Fig. 1. BBR ameliorates hepatic steatosis in HFD-fed rats. The liver isolated from rats fed with a normal diet (ND), high-fat diet (HFD) or high-fat plus Berberine (HFD + BBR) were stain with hematoxylin and eosin. a ND group; (b) HFD group; (c) HFD + BBR group. Photographs are at 100 × magnification. Steatosis shown in blue arrow, necrosis and inflamation shown in red arrows. d NAFLD activity score. Data are presented as mean ± SD from eight rats.**P < 0.01,* P < 0.05.n = 8/group

Fig. 2. Biochemical changes in serum and liver in NAFLD rats. The level of serum ALT, AST, TG, CHOL, FFAs and hepatic TG and CHOL contents. Data are presented as mean ± SD from eight rats.**P < 0.01,* P < 0.05.n = 8/group

Fig. 3. Serum proinflammatory cytokines in NAFLD rats, which were measured by ELISA. a serum CCL2 levels; (b) serum TNF-α levels. Data are presented as mean ± SD from eight rats. **P < 0.01,* P < 0.05.n = 8/group

Fig. 4. Levels of Angptl2, Foxo1, CCR2 and NF-κB-mRNA were measured by real time-PCR and normalized by GAPDH expression; **P < 0.01,* P < 0.05.n = 8/group

Fig. 5. The protein levels of Angptl2, Foxo1, NF-κB, p-NF-κB and CCR2 were measured by Western blot analysis and normalized by GAPDH expression. Data are presented as mean ± SD from eight rats. **P < 0.01,* P < 0.05.n = 8/group