Key result
Exogenous expression of sialidases from Salmonella and Influenza A neuraminidase effectively cleaved alpha (2-3)-sialic acid receptors on cell surfaces in vitro.
Expression of specific sialidases (Salmonella and Influenza A neuraminidase) in cell lines effectively cleaves sialic acid receptors, suggesting a potential genetic strategy to create influenza-resistant poultry.
May enable genetic strategies for influenza-resistant poultry; hypothesis-generating and requires in vivo validation before any application.
Background: Influenza virus can cause both seasonal infections and unpredictable pandemics. Rapidly evolving avian H5N1 virus is getting increasingly infective for humans. Since avian Influenza can be transmitted by domestic birds, serving as a key link between wild aquatic birds and humans, an effective measure to control the influenza transmission would be eradication of the infection in poultry. It is known that the virus penetrates into the cell through binding with the terminal oligosaccharides - sialic acids (SA) - on the cell surfaces. Removal of SA might be a potential antiviral strategy. An approach to developing chicken lines that are resistant to influenza viruses could be the creation of genetically modified birds. Thus it is necessary to select a gene that provides defense to influenza. Here we have expressed in cells a range of exogenous sialidases and estimated their activity and specificity towards SA residues. Methods: Several bacterial, viral and human sialidases were tested. We adopted bacterial sialidases from Salmonella and Actinomyces for expression on the cell surface by fusing catalytic domains with transmembrane domains. We also selected Influenza A/PuertoRico/8/34/H1N1 neuraminidase and human membrane sialidase ( hNeu3 ) genes. Lectin binding assay was used for estimation of a α (2,3)-sialylation level by fluorescent microscopy and FACS. Results: We compared sialidases from bacteria, Influenza virus and human. Sialidases from Salmonella and Influenza A neuraminidase effectively cleaved α (2-3)-SA receptors. Viral neuraminidase demonstrated a higher activity. Sialidases from Actinomyces and hNeu3 did not show any activity against α (2-3) SA under physiological conditions. Conclusion : Our results demonstrated that sialidases with different specificity and activity can be selected as genes providing antiviral defence. Combining chosen sialidases with different activity together with tissue-specific promoters would provide an optimal level of desialilation to prevent infection. Tissue specific expression of the sialidases could protect domestic birds from infection.
No takes yet. Share an insight, caveat, or question.
Antonova et al. (2018) studied Influenza A (in vitro model). Exogenous expression of sialidases (Salmonella typhimurium and Influenza A neuraminidase) vs. Catalytically inactive sialidase was evaluated on Cleavage of alpha (2-3)-sialic acid receptors (measured by lectin binding assay MFI). Exogenous expression of sialidases from Salmonella and Influenza A neuraminidase effectively cleaved alpha (2-3)-sialic acid receptors on cell surfaces in vitro.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: