Key result
Co-inoculation with PCV2 significantly inhibited PRV-induced upregulation of IFN-γ, IL-4, and IL-12p40 mRNA expression in pig peripheral blood mononuclear cells.
PCV2 co-inoculation reduces the cellular immune response to the pseudorabies attenuated vaccine in pig peripheral blood mononuclear cells.
PCV2 co-inoculation may impair PRV vaccine cellular immunity in pigs; leaves open whether co-infections alter swine vaccine efficacy.
We investigated the immune response effects of porcine circovirus type 2 (PCV2) on cells inoculated with pseudorabies attenuated vaccine (PRV). Real-time PCR was used to detect the mRNA expression levels of the regulatory cytokines IL-4, IL-10, IL-12p40, and IFN-γ in pig peripheral blood mononuclear cells, after in vitro single vaccination and co-inoculation with PCV2 and the PRV. We found that PRV causes upregulation of IL-4, IL-12p40, and IFN-γ mRNA expression, while PCV2 causes mRNA upregulation of IL-4, IL-10, and IL-12p40. Moreover, PCV2 inhibited PRV-induced upregulation of IL-4, IL-12p40, and IFN-γ mRNA expression; IFN-γ mRNA expression was significantly inhibited. We conclude that PCV2 can reduce the cellular immune response to PRV.
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Gao et al. (2014) studied this question. Co-inoculation with PCV2 and PRV vs. Single vaccination with PRV was evaluated on mRNA expression levels of IL-4, IL-10, IL-12p40, and IFN-γ. Co-inoculation with PCV2 significantly inhibited PRV-induced upregulation of IFN-γ, IL-4, and IL-12p40 mRNA expression in pig peripheral blood mononuclear cells.
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