Key result
Epitope-tagged chimeric PCV1-2 vaccine viruses were infectious in pigs and elicited both tag-specific antibodies and anti-PCV2 neutralizing antibodies, demonstrating potential as a marker vaccine.
A chimeric porcine circovirus (PCV1-2) with the capsid gene of pathogenic PCV2 cloned into the genomic backbone of nonpathogenic PCV1 is attenuated in pigs but elicits protective immunity against PCV2. In this study, short epitope tags were inserted into the C terminus of the capsid protein of the chimeric PCV1-2 vaccine virus, resulting in a tractable marker virus that is infectious both in vitro and in vivo. Pigs experimentally infected with the epitope-tagged PCV1-2 vaccine viruses produced tag-specific antibodies, as well as anti-PCV2 neutralizing antibodies, indicating that the epitope-tagged viruses could potentially serve as a positive-marker modified live-attenuated vaccine.
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Beach et al. (2011) studied Healthy pigs (Porcine circovirus vaccine model) (n=12). Epitope-tagged chimeric PCV1-2 vaccine viruses (PCV1-2-KT3, PCV1-2-GLU) vs. Wild-type PCV1-2 and PBS was evaluated on Infectivity (viremia) and induction of anti-epitope tag and anti-PCV2 neutralizing antibodies. Epitope-tagged chimeric PCV1-2 vaccine viruses were infectious in pigs and elicited both tag-specific antibodies and anti-PCV2 neutralizing antibodies, demonstrating potential as a marker vaccine.
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