Kinetics of Thermal Denaturation of β-Lactoglobulin at pH 2.5

denaturation of /~-lactoglobulin at pH 2.5 was studied at temperatures ranging from 60 to 130 C. Changes were followed by measurements of specific optical rotation [0d2ss9 and solubility at pH 4.5. Denaturation, as determined by both methods, was irreversible and was evident only at 75 C or above, a higher temperature than 65 C reported for denaturation at neutral pH. Also, the cooperative transition temperatures were higher (83 to 84 C) at pH 2.5 than at pH 6.7 (68 to 69 C).