A method of successfully inducing experimental ketosis in lactation dairy cows within nine weeks postpartum was developed. Animals were on a low-energy ration providing 90 % of their protein and 30% of their energy requirements for 8 days. On the fourth through seventh days the cows were injected with L-thyroxine (sodium) pentahydrate at a daily dosage of 6.9 mg per 100 kg of initial body weight. The animals exhibited typical bovine ketotie signs of lethargy or excitability or both, dull eyes, anorexia, decreased milk production, and increased fat percentage. Blood glucose decreased to a mean of 15.4 mg per 100 ml and total ketones, acetone, and free fatty acids increased to means of 34.3, 26.2, and 10.7 mg per 100 ml.
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Kellogg et al. (1971) studied this question.
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