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March 25, 2009Avian PathologyOpen Access

Quantification of pigeon circovirus in serum, blood, semen and different tissues of naturally infected pigeons using a real-time polymerase chain reaction

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Key result

A newly developed real-time PCR assay successfully quantified pigeon circovirus DNA across various tissues, revealing high viral loads in the bursa of Fabricius, liver, and spleen of sick pigeons.

Population

Naturally infected pigeons, including those with 'young pigeon sickness' and apparently healthy pigeons, as…

Design

Preclinical

Authors

JDJ.P. DuchatelDTD. ToddUniversity of North Carolina at GreensboroCWC. Willeman

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Overview

May aid circovirus diagnostics in pigeons; leaves open standardization and clinical validation in avian practice.

Structured PICO

P
Population
Naturally infected pigeons, including those with 'young pigeon sickness' and apparently healthy pigeons, as well as historical sera samples from Belgium (1991).
E
Exposure
Real-time polymerase chain reaction (PCR) based on SYBR Green chemistry for quantification of pigeon circovirus (PiCV) DNA
O
Outcome
Quantification of pigeon circovirus (PiCV) DNA (viral load) in serum, blood, semen, cloacal swabs, and tissues (liver, bursa, spleen)surrogate

A newly developed real-time PCR assay effectively quantifies pigeon circovirus DNA across various tissues and fluids, revealing high viral loads in both sick and some healthy pigeons and confirming potential vertical and horizontal transmission routes.

Cite This Study

Duchatel et al. (2009) studied Pigeon circovirus (PiCV) infection. Real-time PCR based on SYBR Green chemistry was evaluated on Detection and quantification of PiCV DNA. A newly developed real-time PCR assay successfully quantified pigeon circovirus DNA across various tissues, revealing high viral loads in the bursa of Fabricius, liver, and spleen of sick pigeons.

synapsesocial.com/papers/6a964fcb31394ac286fbaed9https://doi.org/10.1080/03079450902737805
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Also Consider

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