The inhibition of growth of vaccinia and cowpox viruses in RK 13 cells

SUMMARY The growth of vaccinia and cowpox in RK13 cells was studied by measurement of infective virus production, gel diffusion and haemagglutination. The effects of virus growth inhibitors on the normal course of infection were then followed. Although the results with the two viruses were essentially similar some differences were detected between them. Inhibitors of DNA synthesis permitted production of many virus-specific soluble antigens. Compounds which inhibit DNA function and compounds that affected energy-yielding reactions had more dramatic effects. The different results obtained suggested that the synthesis of virus-specific materials was sequential and a possible part of the sequence is suggested.