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March 25, 2020AJP Cell Physiology

Role of complement 3 in renin generation during the differentiation of mesenchymal stem cells to smooth muscle cells

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Key result

Complement 3 maintains the undifferentiated state of smooth muscle cells from mesenchymal stem cells to generate renin, contributing to hypertension pathogenesis in spontaneously hypertensive rats.

Why the study?

To investigate the contributions of complement 3 to the development of hypertension pathogenesis by evaluating renin-producing cell formation and renin generation during differentiation of primary bone marrow-mesenchymal stem cells to smooth muscle cells.

Population

Primary bone marrow-MSCs from C57BL/6 mice, WKY rats, and SHRs

Comparison

Differentiation to SMCs with TGF-beta1, exogenous C3a, or C3a receptor antagonist SB290157 vs controls

Design

Preclinical in vitro experimental study

Authors

LCLan ChenNFNoboru FukudaSSShoichi Shimizu

Discussion

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Member takes

Overview

C3 may promote hypertensive remodeling via local RAS in rats; leaves open human renin regulation and therapeutic targeting.

Structured PICO

P
Population
Primary bone marrow-mesenchymal stem cells (MSCs) from C57BL/6 mice, Wistar-Kyoto (WKY) rats, and spontaneously hypertensive rats (SHRs)
E
Exposure
Differentiation to smooth muscle cells with transforming growth factor-β1, exogenous C3a, and C3a receptor antagonist SB290157
C
Comparator
Untreated cells and cells from normotensive WKY rats
O
Outcome
Renin generation and expression of LXRα, C3, and KLF5 during differentiationsurrogate

Complement 3 plays a key role in maintaining an undifferentiated state in smooth muscle cells derived from mesenchymal stem cells, promoting renin generation that may contribute to the pathogenesis of hypertension.

Cite This Study

Chen et al. (2020) studied Hypertension. Complement 3 (C3) was evaluated on Renin generation during differentiation of MSCs to SMCs. Complement 3 maintains the undifferentiated state of smooth muscle cells from mesenchymal stem cells to generate renin, contributing to hypertension pathogenesis in spontaneously hypertensive rats.

synapsesocial.com/papers/6a96604d672df19136a6b618https://doi.org/10.1152/ajpcell.00461.2019
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Involvement of complement 3 in the salt-sensitive hypertension by activation of renal renin-angiotensin system in spontaneously hypertensive rats2018 · 30 citations
  2. 2Local Renin Angiotensin Expression Regulates Human Mesenchymal Stem Cell Differentiation to Adipocytes2006 · 115 citations
  3. 3The Morphology of Juxtaglomerular Cell Hyperplasia and Hypertrophy in Normotensive Rats and Monkeys Given an Angiotensin II Receptor Antagonist1995 · 28 citations
  4. 4Multilineage Potential of Adult Human Mesenchymal Stem Cells1999 · 21,220 citations
  5. 5LXRα functions as a cAMP-responsive transcriptional regulator of gene expression2000 · 81 citations