What question did this study set out to answer?

To determine whether expressing virus-derived hairpin double-stranded RNA in transgenic tobacco induces stable, inheritable immunity against Tobacco mosaic virus and Cucumber mosaic virus.

January 27, 2011Virology JournalOpen Access

Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus

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To determine whether expressing virus-derived hairpin double-stranded RNA in transgenic tobacco induces stable, inheritable immunity against Tobacco mosaic virus and Cucumber mosaic virus.
Cloned inverted repeats of partial TMV movement protein (MP) and CMV replication protein (Rep) genes into plant expression vectors and transformed them into tobacco via Agrobacterium tumefaciens.
Assayed viral resistance in T₄ progeny under standard and low temperature (15 °C) conditions, assessing transgene copy number effects and co-infection resistance.
Generated three transgenic lines completely immune to TMV (MP16-17-3, MP16-17-29, MP16-17-58) and three lines completely immune to CMV (Rep15-1-1, Rep15-1-7, Rep15-1-32).
Viral immunity was stably inherited through the T₄ generation, persisted at 15 °C, showed no significant correlation with transgene copy number, and resisted compromise by CMV co-infection.

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Implication

Experimental study demonstrates complete viral immunity in transgenic tobacco expressing hairpin RNA, indicating stable RNA-silencing protection against mosaic viruses.

Cite This Study

Hu et al. (2011) studied this question.

synapsesocial.com/papers/6a9660d9a45595e5080e1e1e https://doi.org/10.1186/1743-422x-8-41

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Implication

Experimental study demonstrates complete viral immunity in transgenic tobacco expressing hairpin RNA, indicating stable RNA-silencing protection against mosaic viruses.

Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus

Key Points

Discussion

Implication

Cite This Study

Virus-derived transgenes expressing hairpin RNA give immunity to Tobacco mosaic virus and Cucumber mosaic virus

Key Points

Discussion

Implication

Cite This Study

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