What question did this study set out to answer?

To evaluate a reproducible, inexpensive method for isolating fetal cells from maternal blood to enable noninvasive prenatal detection of trisomies 18 and 21.

September 10, 1993American Journal of Reproductive Immunology

Detection of Fetal Trisomies 21 and 18 From Maternal Blood Using Triple Gradient and Magnetic Cell Sorting

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Authors

DGDorothee Gänshirt-AhlertUniversity of Münster RBRegina BÖRJESSON‐STOLLUniversity of Münster MBMonika BurschykUniversity of Münster

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Implication

Diagnostic study demonstrates reliable identification of fetal trisomies 21 and 18 from maternal blood using magnetic cell sorting, indicating viable noninvasive prenatal screening.

Key Points

To evaluate a reproducible, inexpensive method for isolating fetal cells from maternal blood to enable noninvasive prenatal detection of trisomies 18 and 21.
Enriched fetal cells from maternal blood samples using triple density gradient separation followed by magnetic-activated cell sorting (MACS) directed at anti-CD71 transferrin receptor antibodies.
Performed chromosome-specific fluorescence in situ hybridization (FISH) to detect fetal aneuploidy across 15 confirmed cases (10 trisomy 21, 5 trisomy 18) and normal pregnancy controls.
In all 15 trisomy pregnancies, the percentage of isolated cells exhibiting three hybridization signals showed zero overlap with normal control samples.
Successful distinction of aneuploid cases occurred across varying gestational ages and independently of whether patients had undergone previous invasive procedures.

Cite This Study

Gänshirt-Ahlert et al. (1993) studied this question.

synapsesocial.com/papers/6a966fa3a45595e5080e2244 https://doi.org/10.1111/j.1600-0897.1993.tb00620.x

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Implication

Diagnostic study demonstrates reliable identification of fetal trisomies 21 and 18 from maternal blood using magnetic cell sorting, indicating viable noninvasive prenatal screening.

Detection of Fetal Trisomies 21 and 18 From Maternal Blood Using Triple Gradient and Magnetic Cell Sorting

Discussion

Member takes

Implication

Key Points

Cite This Study

Detection of Fetal Trisomies 21 and 18 From Maternal Blood Using Triple Gradient and Magnetic Cell Sorting

Discussion

Member takes

Implication

Key Points

Cite This Study

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