H. D. Baernstein, C. W. Rees, I. L. Bartgis, The Rate of Reproduction of Entamoeba histolytica in Microcultures from Inocula of Single Trophozoites in Cell-Free Medium Prepared from Embryos of the Chick, The Journal of Parasitology, Vol. 43, No. 2 (Apr., 1957), pp. 143-152
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Baernstein et al. (1957) studied this question.
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