What question did this study set out to answer?

To identify and characterize novel antibiotic resistance genes from complex soil microbial communities using functional metagenomics.

January 31, 2011Environmental Microbiology

Characterization of novel antibiotic resistance genes identified by functional metagenomics on soil samples

View Full Paper

Ask AI

Bookmark

Authors

GTGloria Torres‐CortésLight Prescriptions Innovators (Spain)VMVicenta MillánEstación Experimental del Zaidín HRHugo Ramírez-SaadUniversidad Autónoma Metropolitana

Discussion

Member takes

Implication

Functional metagenomic study uncovers 11 novel antibiotic resistance genes in soil microbiomes, highlighting soil as an uncharacterized reservoir of resistance determinants.

Key Points

To identify and characterize novel antibiotic resistance genes from complex soil microbial communities using functional metagenomics.
Constructed functional metagenomic libraries from three distinct soil samples comprising 3.6 Gb of total DNA.
Screened clones for antibiotic resistance and heterologously expressed candidate genes in Escherichia coli and Sinorhizobium meliloti to measure enzymatic activity and kinetics.
Identified 11 novel antibiotic resistance genes conferring resistance to ampicillin (n=3), gentamicin (n=2), chloramphenicol (n=2), and trimethoprim (n=4).
Characterized a novel 26.8 kDa trimethoprim resistance enzyme (Tm8-3) with dihydrofolate reductase activity exhibiting a Km of 8.9 µM for NADPH, a Km of 3.7 µM for dihydrofolate, and an IC50 of 20 µM for trimethoprim.

Cite This Study

Torres‐Cortés et al. (2011) studied this question.

synapsesocial.com/papers/6a96798037dc33ab6afbb7ac https://doi.org/10.1111/j.1462-2920.2010.02422.x

View Full Paper

Ask AI

Bookmark

Implication

Functional metagenomic study uncovers 11 novel antibiotic resistance genes in soil microbiomes, highlighting soil as an uncharacterized reservoir of resistance determinants.

Characterization of novel antibiotic resistance genes identified by functional metagenomics on soil samples

Discussion

Member takes

Implication

Key Points

Cite This Study

Characterization of novel antibiotic resistance genes identified by functional metagenomics on soil samples

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider